Cell-free DNA Preservation Solution for Blood Collection Tube

Technique：Use EDTA-K3 as anticoagulant, which has highest solubility in the liquid, to bind to Ca²⁺ ions in blood to form chelates, and inhibit thrombospondin activation to prevent blood clotting.

Production：Full set of reagents are manufactured in GMP production factory with ISO13485 standard; control the source production process, use scientific verification system with industry gold standard.

Application：Thousands of clinical samples tested in the NGS testing laboratory of Geneseeq (CAP & CLIA certified), with stable results and expected performance indicators.

Company Information

BunnyTeeth Technology Inc. is committed to building a complete core technology platform for bioengineering, building a high-quality biotech product chain and a technical service chain, with the ambition to become a global leader in molecular and genetic diagnostic. BunnyTeeth Technology Inc. will also provide customers with a full-range of one-step service solution in the field of molecular diagnostics and genetic diagnostic.

Production Information

Produciton Description

The role of plasma cell-free DNA (cfDNA) is becoming increasingly evident as an important test sample for tumor diagnosis and non-invasive prenatal screening. Cell-free DNA Preservation Solution for Blood Collection Tube can be directly used for whole blood collection, preservation and transportation, which can effectively protect cfDNA from degradation by nucleases and avoid the release of genomic DNA from blood nucleated cells, greatly ensuring the downstream application of cfDNA.

Product Advantages

1.  High Stability: Effectively avoids cfDNA degradation and genomic DNA release.
2.  High Tolerance: Good temperature tolerance for easy storage and transport of samples.
3.  Excellent Sequencing: The extracted cfDNA has excellent library preparation efficiency and sequencing quality.
4.  Wide Application: Designed for liquid biopsies for oncology, infection, prenatal diagnosis, etc.

Experimental Data

Figure 1. Hemolysis of blood samples and stable cfDNA yield at different storage times.

Blood samples were collected into 3 brands of preservation solutions, and the samples were placed at room temperature for 0, 3, 7 and 14 days to compare the hemolysis and cfDNA yield. Cell-free DNA Preservation Solution for Blood Collection Tube could effectively inhibit hemolysis and the extracted cfDNA yield was stable.

Figure 2. Hemolysis of blood samples and stable cfDNA yield at different transport temperatures.

The blood samples were collected into 3 brands of preservation solutions, and samples were compared for hemolysis and cfDNA yield after 0 days at room temp, 3 days at room temp (25℃), high temp (35℃) and low temp (4℃) transportation. Cell-free DNA Preservation Solution for Blood Collection Tube can withstand different transportation temperatures and have better resistance to genomic nucleic acid release.

Product Component

Storage Conditions

Store at 10-30°C upon arrival (not freeze), for up to 12 months.

Application Field

1. Fetal non-invasive birth defect screening (non-invasive Down's syndrome test)
2. Tumor biomarker discovery and research
3. Oncology treatment and efficacy assessment studies
4. Clinical drug development and testing
5. Related disease molecular level research